Immortalized Prostate Fibroblast Growth Medium

Catalog Number: IM01 
Product Format: Frozen or Liquid
Product Volume: 500 mL
Product Usage: Research Use Only. Not for Clinical Use.

Notes

Custom formulations available:

• Serum-free (with human platelet lysate)

• CAF-conditioned (for tumor microenvironment modeling)

Product Description

A complete, optimized growth medium designed for the culture and expansion of immortalized human prostate fibroblasts (e.g., WPMY-1, BHPrS1). This formulation supports stable growth while preserving characteristic fibroblast morphology and biomarker expression (FSP-1, α-SMA, vimentin). It is ideal for applications involving prostate stromal modeling, CAF studies, and co-culture systems with cancer or epithelial cells.

Physicochemical Properties

• pH: 7.2–7.4

• Osmolality: 300–320 mOsm/kg

• Color: Red (contains phenol red indicator)

Key Features

✔ Validated for prostate fibroblast lines (e.g., WPMY-1 doubling time: 24–30 hrs)
✔ Maintains stromal marker expression:

• Vimentin+ (>95%)

• FSP-1+ (>90%)

• α-SMA+ (variable)
  ✔ Promotes ECM secretion (collagen I/III, fibronectin)
  ✔ Pre-tested for mycoplasma, viruses, and sterility
  ✔ Compatible with 3D culture models (e.g., Matrigel, collagen scaffolds)

Storage & Handling

• Storage Temp: 2–8°C (liquid) or –20°C (long-term frozen)

• Shelf Life:

  • Unopened: 12 months at 2–8°C

  • After opening: 4 weeks at 2–8°C

• Note: Avoid repeated freeze-thaw cycles

Recommended Culture Protocol

Subculture Procedure

1. Wash cells with Ca²⁺/Mg²⁺-free PBS

2. Detach using cell detachment solution

3. Neutralize trypsin with neutralization solution

4. Centrifuge at 200 × g for 5 minutes

5. Resuspend in fresh IPFGM002 medium and seed at 5,000–10,000 cells/cm² in T25 or T75 flasks

Feeding Schedule

• Change medium every 48–72 hours

• Passage cells at 70–80% confluency (avoid overgrowth)

Quality Control – Performance Cell Data

Safety Testing

• Sterility: 14-day incubation — No microbial growth

• Mycoplasma: PCR — Negative

Troubleshooting Guide

Ordering Information

References

• Webber et al. (1999). Prostate — WPMY-1 Cell Line Characterization

• Barron & Rowley (2012). Nature Reviews Urology — Prostate Stroma Biology

Related Products:

• Prostate Fibroblast Cryopreservation Medium

• Fibroblast Activation Marker Antibodies

Product Usage

Cells are offered for Research Use Only. Not for Clinical Use.