Immortalized Bronchial Fibroblast Growth Medium

Catalog Number: IM05 
Product Format: Frozen or Liquid
Product Volume: 500 mL
Product Usage: Research Use Only. Not for Clinical Use.

Notes

• For hypoxic conditions: Reduce serum to 5% and add 100 µM CoCl₂.

• Custom formulations available: serum-free, low-glucose versions available upon request.

Product Description

A complete, serum-supplemented medium optimized for the proliferation and maintenance of immortalized human bronchial fibroblasts or similar cell lines. Supports high cell viability, consistent growth rates, and long-term retention of the spindle-shaped fibroblast phenotype.

Physicochemical Properties

• pH: 7.2–7.4

• Osmolality: 290–310 mOsm/kg

• Color: Red (phenol red indicator)

Key Features

✔ High proliferation rates (doubling time: ~24–36 hours)
✔ Maintains fibroblast morphology (spindle-shaped, α-SMA⁺ expression)
✔ Low endotoxin content (<0.5 EU/mL)
✔ Pre-tested for mycoplasma contamination

Storage & Handling

• Liquid Storage: 2–8°C

• Frozen Storage: –20°C (for long-term)

• Shelf Life:

  • Unopened: 12 months at 2–8°C

  • After opening: 4 weeks at 2–8°C

• Note: Avoid repeated freeze-thaw cycles

Recommended Culture Protocol

Subculture Procedure

1. Wash cells with Ca²⁺/Mg²⁺-free PBS

2. Detach using cell detachment solution

3. Neutralize trypsin with neutralization solution

4. Centrifuge at 200 × g for 5 min

5. Resuspend in fresh growth medium and seed at 5,000–10,000 cells/cm² in T25 or T75 flasks

Feeding Schedule

• Change medium every 48–72 hours

• Passage at 70–80% confluency (avoid overgrowth)

Quality Control

Safety Testing

• Sterility: 14-day culture, no microbial growth

• Mycoplasma (PCR): Negative

Troubleshooting

Ordering Information

References

• Smith et al. (2022). Optimized Culture of Bronchial Fibroblasts for Airway Remodeling Studies. Cell Biol. Rep.

• Related Products

  • Bronchial Fibroblast Cryopreservation Medium

  • Fibroblast Activation Marker Antibodies

Product Usage

Cells are offered for Research Use Only. Not for Clinical Use.