Name of Products
Human Uterine Microvascular Endothelial Cells
Catalog Number
HU07
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
Primary endothelial cells isolated from normal human uterine tissue, designed for research in angiogenesis, hormonal regulation, and reproductive microvascular biology. Cells are provided at passage 1 and shipped frozen. For optimal cell performance, use Nuvyra Labs Endothelial Growth Medium.
Storage Conditions
-
Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)
-
Shipping Temperature: −80°C
-
Stability: Very stable in appropriate cryogenic storage
Cell Characterization / Specification
Quality Control Results
| Parameter |
Method |
Acceptance Criteria |
Result |
| Sterility – Bacteria |
Growth Plate |
Negative |
Negative |
| Sterility – Fungi |
Growth Plate |
Negative |
Negative |
| Endotoxin Level |
Kinetic LAL |
≤1.0 EU/μg |
< 0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / HBV |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
> 5 × 10⁵ |
5.0 × 10⁵ |
| Viability |
Trypan Blue |
>95% viable |
97% |
Handling Upon Arrival
Immediately transfer frozen vials into a −80°C freezer for short-term storage or to a liquid nitrogen tank for long-term storage upon receipt.
Thawing & Subculturing Instructions
A) Pre-coat a T25 flask with 2 mL Coating Solution, incubate 5 min, rinse with 4 mL rinse solution, and aspirate to prepare the flask.
B) Thaw the vial in a 37°C water bath, disinfect, and transfer to a biosafety cabinet. Seed into flask with 10 mL Endothelial Growth Medium.
C) Once confluent, rinse twice with 5 mL rinse solution, add 2 mL Detachment Solution, aspirate after 20 sec.
D) Allow detachment (1–2 min), confirm under microscope, then tap flask to release cells.
E) Add 5 mL Neutralization Solution, centrifuge at 800g for 5 min.
G) Resuspend in 10–15 mL medium and split into 2–3 flasks (1:2 or 1:3 ratio).
H) Change medium every 2–3 days; confluence typically achieved within 7 days.
I) For quiescence, switch to Endothelial Basal Medium ~12 hrs prior to experiments.
Cells are offered for Research Use Only. Not for Clinical Use.
