Name of Products
Human Umbilical Vein Endothelial Cells Expressing VE-CAD-GFP
Catalog Number
HUV9
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
Human Umbilical Vein Endothelial Cells (HUVECs) were isolated from normal human umbilical cord tissue. These cells were genetically modified via lentiviral transduction to stably express VE-Cadherin-GFP, followed by puromycin selection. This allows live visualization of cell junction dynamics and vascular integrity in real time. Cells are provided at passage 1 and shipped frozen. We recommend using Nuvyra Labs Endothelial Growth Medium for optimal cell performance.
Storage Conditions
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Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)
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Shipping Temperature: −80°C
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Stability: Very stable under proper cryogenic conditions
Cell Characterization / Specification
Quality Control Results
| Parameter |
Method |
Acceptance Criteria |
Result |
| Sterility – Bacteria |
Growth Plate |
Negative |
Negative |
| Sterility – Fungi |
Growth Plate |
Negative |
Negative |
| Endotoxin Level |
Kinetic LAL |
≤1.0 EU/μg |
< 0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / HBV |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
> 5 × 10⁵ |
5.0 × 10⁵ |
| Viability |
Trypan Blue |
>95% viable |
97% |
Handling Upon Arrival
Transfer vials immediately to a −80°C freezer for short-term storage or to a liquid nitrogen tank for long-term preservation upon delivery.
Thawing & Subculturing Instructions
A) Pre-coat a T25 flask with 2 mL Coating Solution, incubate for 5 min, rinse with 4 mL rinse solution, and aspirate to prepare.
B) Thaw the vial in a 37°C water bath, disinfect the exterior with 70% ethanol, and open in a biosafety cabinet. Seed into flask with 10 mL Endothelial Growth Medium.
C) Once confluent, rinse twice with 5 mL rinse solution, add 2 mL Detachment Solution, aspirate excess after 20 seconds.
D) Incubate at RT or 37°C for 1–2 min until cells round up; tap flask gently to dislodge cells.
E) Add 5 mL Neutralization Solution, centrifuge at 800g for 5 min.
G) Resuspend pellet with 10–15 mL medium and split into 2–3 pre-coated flasks.
H) Refresh medium every 2–3 days; confluence typically reached in 7 days at 1:3 ratio.
I) For quiescent conditions, switch to Basal Medium for 12 hrs prior to experiments.
Cells are offered for Research Use Only. Not for Clinical Use.
