Name of Products
Human Umbilical Vein Endothelial Cells
Catalog Number
HU21
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
Human Umbilical Vein Endothelial Cells (HUVECs) were isolated from healthy human umbilical cord tissue. These primary endothelial cells are commonly used in vascular biology studies, including angiogenesis, permeability, and inflammatory responses. Cells are cryopreserved at passage 1 and shipped in frozen vials. Use of Nuvyra Labs Endothelial Growth Medium is recommended for optimal performance.
Storage Conditions
-
Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)
-
Shipping Temperature: −80°C
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Stability: Stable when stored appropriately in the vapor phase of liquid nitrogen
Cell Characterization / Specification
Quality Control Results
| Parameter |
Method |
Acceptance Criteria |
Result |
| Sterility – Bacteria |
Growth Plate |
Negative |
Negative |
| Sterility – Fungi |
Growth Plate |
Negative |
Negative |
| Endotoxin Level |
Kinetic LAL |
≤1.0 EU/μg |
< 0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / HBV |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
> 5 × 10⁵ |
5.0 × 10⁵ |
| Viability |
Trypan Blue |
>95% viable |
97% |
Handling Upon Arrival
Upon receiving the dry ice shipment, immediately store frozen vials in a −80°C freezer (short term) or transfer to liquid nitrogen (long term).
Thawing & Subculturing Instructions
A) Pre-coat T25 flasks with 2 mL Coating Solution, incubate for 5 min, rinse with 4 mL Rinse Solution, and remove.
B) Thaw cells in a 37°C water bath, sanitize vial with 70% ethanol, and work in a biosafety cabinet. Add thawed contents to a pre-coated T25 flask with 10 mL Endothelial Growth Medium.
C) When confluent, rinse twice with 5 mL Rinse Solution, add 2 mL Detachment Solution, and aspirate excess within 20 seconds.
D) Incubate at RT or 37°C for ~1–2 min until cells round up. Tap gently to detach cells.
E) Add 5 mL Neutralization Solution and centrifuge at 800g for 5 min.
G) Resuspend in 10–15 mL medium and seed 5 mL/flask into 2–3 flasks (1:2 or 1:3 ratio).
H) Replace medium every 2–3 days. Expect confluence in ~7 days.
I) To prepare quiescent cells, switch to Basal Medium for ~12 hours before experiments.
Cells are offered for Research Use Only. Not for Clinical Use.
