Name of Products
Human Umbilical Artery Endothelial Cells
Catalog Number
HUA1
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
Primary endothelial cells derived from normal human umbilical artery tissue, shipped frozen at passage 1. Ideal for studies in vascular development, angiogenesis, and endothelial function. For optimal results, we recommend culturing with Nuvyra Labs Endothelial Growth Medium.
Storage Conditions
-
Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)
-
Shipping Temperature: −80°C
-
Stability: Very stable under proper cryogenic storage conditions
Cell Characterization / Specification
Quality Control Results
| Parameter |
Method |
Acceptance Criteria |
Result |
| Sterility – Bacteria |
Growth Plate |
Negative |
Negative |
| Sterility – Fungi |
Growth Plate |
Negative |
Negative |
| Endotoxin Level |
Kinetic LAL |
≤1.0 EU/μg |
< 0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / HBV |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
> 5 × 10⁵ |
5.0 × 10⁵ |
| Viability |
Trypan Blue |
>95% viable |
97% |
Handling Upon Arrival
Transfer frozen vials immediately to −80°C (short term) or liquid nitrogen (long term). Handle with appropriate PPE.
Thawing & Subculturing Instructions
A) Pre-coat T25 flask with 2 mL Coating Solution, incubate 5 min, rinse with 4 mL rinse solution, and aspirate.
B) Thaw vial in a 37°C water bath, disinfect, and transfer to biosafety cabinet.
C) Add 10 mL Endothelial Growth Medium to flask and seed cells.
D) Once confluent, rinse flask twice with 5 mL rinse solution, add 2 mL Detachment Solution, aspirate after 20 seconds.
E) Wait 1–2 minutes at RT or 37°C, then tap flask to dislodge cells.
F) Add 5 mL Neutralization Solution, centrifuge at 800g for 5 min.
G) Resuspend pellet in 10–15 mL medium, seed into 2–3 flasks (1:2 to 1:3 split).
H) Change medium every 2–3 days; cells typically reach confluence within 7 days.
I) For quiescent cells, switch to Endothelial Basal Medium for 12 hrs before experiments.
Cells are offered for Research Use Only. Not for Clinical Use.
