Name of Products
Human Small Intestine Microvascular Endothelial Cells
Catalog Number
HS10
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
These cells were isolated from normal human small intestinal tissue, ideal for research into gut vascular biology, barrier function, and microcirculatory regulation. Shipped as passage 1 cryopreserved cells. For optimal growth and phenotype maintenance, use Nuvyra Labs Endothelial Growth Medium.
Storage Conditions
-
Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)
-
Shipping Temperature: −80°C
-
Stability: Very stable in cryostorage under vapor phase nitrogen
Cell Characterization / Specification
Quality Control Results
| Parameter |
Method |
Acceptance Criteria |
Result |
| Sterility – Bacteria |
Growth Plate |
Negative |
Negative |
| Sterility – Fungi |
Growth Plate |
Negative |
Negative |
| Endotoxin Level |
Kinetic LAL |
≤1.0 EU/μg |
< 0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / HBV |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
> 5 × 10⁵ |
5.0 × 10⁵ |
| Viability |
Trypan Blue |
>95% viable |
97% |
Handling Upon Arrival
Immediately store vials at −80°C for short-term or in liquid nitrogen for long-term preservation.
Thawing & Subculturing Instructions
A) Coat T25 flasks with 2 mL Nuvyra Labs Coating Solution, incubate for 5 minutes, rinse with 4 mL cell rinse solution, aspirate, and flask is ready.
B) Thaw the vial in a 37°C water bath, disinfect with ethanol, and transfer into a biosafety cabinet.
C) Add 10 mL of Endothelial Growth Medium to a pre-coated T25 flask and seed the cells.
D) When confluent, rinse twice with 5 mL rinse solution, add 2 mL Detachment Solution, aspirate excess within 20 seconds.
E) Wait 1–2 minutes at RT or 37°C, then gently tap the flask to detach cells.
F) Add 5 mL Neutralization Solution, centrifuge at 800g for 5 min.
G) Resuspend pellet in 10–15 mL medium, split into 2–3 T25 flasks.
H) Change medium every 2–3 days; confluence expected within 7 days.
I) For quiescent cells, replace with Endothelial Basal Medium for 12 hrs before use.
Cells are offered for Research Use Only. Not for Clinical Use.
