Name of Products
Human Retinal Microvascular Endothelial Cells
Catalog Number
HRM2
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
Cells were isolated from normal human retinal microvascular tissue, ideal for ophthalmologic and microvascular research. Cells are provided at passage 1 and shipped in cryovials. For optimal growth and morphology, use Nuvyra Labs Endothelial Growth Medium.
Storage Conditions
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Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)
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Shipping Temperature: −80°C
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Stability: Very stable under proper cryostorage
Cell Characterization / Specification
Quality Control Summary
| Parameter |
Method of Determination |
Acceptance Criteria |
Result |
| Sterility – Bacteria |
Growth Plate |
Negative |
Negative |
| Sterility – Fungi |
Growth Plate |
Negative |
Negative |
| Endotoxin Level (EU/μg) |
Kinetic LAL |
≤ 1.0 EU/μg |
< 0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / HBV (PCR) |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
> 5 × 10⁵ |
5.0 × 10⁵ |
| Viability |
Trypan Blue |
>95% viable |
97% |
Handling Upon Arrival
Upon delivery on dry ice, immediately store in −80°C for short term or in liquid nitrogen for long-term use. Do not thaw unless preparing for culture.
Thawing & Subculturing Instructions
A) Pre-coat a T25 flask with 2 mL Nuvyra Labs Coating Solution, incubate 5 min, rinse with 4 mL cell rinse solution, remove and discard rinse.
B) Thaw vial in 37°C water bath, disinfect, and transfer to biosafety cabinet.
C) Add 10 mL Endothelial Growth Medium to the flask and seed cells.
D) When confluent, subculture:
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Rinse cells with 5 mL rinse solution ×2
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Add 2 mL Detachment Solution, aspirate excess
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Wait 1–2 min at RT or 37°C, tap flask to dislodge cells
-
Add 5 mL Neutralization Solution, centrifuge at 800g for 5 min
E) Resuspend pellet in 10–15 mL medium, split into 2–3 pre-coated flasks
F) Change medium every 2–3 days; cells reach confluence in ~7 days
G) To induce quiescence, replace with Endothelial Basal Medium for 12 hrs before experiment
Cells are offered for Research Use Only. Not for Clinical Use.
