Human Pulmonary Microvascular Endothelial Cells

Name of Products

Human Pulmonary Microvascular Endothelial Cells

Catalog Number

HPM5

Product Format

Frozen Vial

Cell Number

5 × 10⁵ cells/vial

Product Description

Human Pulmonary Microvascular Endothelial Cells were isolated from normal human lung tissue, offering a physiologically relevant model for studying pulmonary vascular function, barrier permeability, and inflammation. Shipped as passage 1 cells in frozen format. Nuvyra Labs Endothelial Growth Medium is strongly recommended to ensure optimal growth and viability.

Storage Conditions

• Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)

• Shipping Temperature: −80°C

• Stability: Very stable when stored properly

Cell Characterization / Specification

• VWF/Factor VIII: >95% positive (cytoplasmic, immunofluorescence)

• Di-I-Ac-LDL Uptake: >95% positive (cytoplasmic)

• PECAM1: >95% positive (cytoplasmic)

Quality Control Summary

Handling Upon Arrival

• Store immediately in a −80°C freezer or liquid nitrogen tank

• Do not thaw unless ready for culture

Thawing & Subculturing Instructions

A) Pre-coat T25 flask with 2 mL Nuvyra Labs Coating Solution, incubate 5 min, rinse with 4 mL cell rinse solution.
B) Thaw vial in 37°C water bath, disinfect exterior with 70% ethanol, transfer contents into biosafety cabinet.
C) Seed into flask with 10 mL Endothelial Growth Medium.
D) For passaging:

• Rinse with 5 mL rinse solution (×2)

• Add 2 mL Detachment Solution, aspirate excess in 20 seconds

• Wait 1–2 min at RT or 37°C

• Tap flask to detach

• Neutralize with 5 mL Neutralization Solution

• Centrifuge at 800g × 5 min

E) Resuspend and seed into 2–3 pre-coated flasks (1:2 or 1:3 ratio).
F) Medium change every 2–3 days.
G) For quiescent cells, switch to Endothelial Basal Medium for 12 hrs before experiments.

Cells are offered for Research Use Only. Not for Clinical Use.