Name of Products
Human Pancreatic Endothelial Cells
Catalog Number
HP12
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
These endothelial cells were isolated from normal human pancreatic tissue. They offer a physiologically relevant model for research into pancreatic vascular biology, diabetes, and endocrine/exocrine interface studies. Cells are shipped at passage 1 in cryopreserved format. We recommend culturing these cells with Nuvyra Labs Endothelial Growth Medium for optimal viability and performance.
Storage Conditions
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Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)
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Shipping Temperature: −80°C
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Stability: Long-term stability under proper cryogenic storage
Cell Characterization / Specification
Quality Control Summary
| Parameter |
Method |
Acceptance Criteria |
Result |
| Sterility – Bacteria |
Growth Plate |
Negative |
Negative |
| Sterility – Fungi |
Growth Plate |
Negative |
Negative |
| Endotoxin Level |
Kinetic LAL |
≤1.0 EU/μg |
<0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / HBV (PCR) |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
>5 × 10⁵ |
5.0 × 10⁵ |
| Viability |
Trypan Blue |
>95% viable |
97% |
Handling Instructions Upon Arrival
Store vials in a −80°C freezer for short-term or liquid nitrogen for long-term preservation immediately upon arrival.
Thawing & Subculturing Protocol
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Pre-coat T25 flask
Add 2 mL of Nuvyra Labs Coating Solution, incubate 5 min, aspirate, and rinse with 4 mL cell rinse solution.
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Thawing
Warm vial in 37°C water bath, spray with 70% ethanol, wipe down, and transfer cells inside biosafety cabinet.
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Initial Seeding
Add 10 mL Nuvyra Labs Endothelial Growth Medium to flask and transfer the thawed cells.
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Passaging
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Rinse with 5 mL rinse solution twice
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Add 2 mL Detachment Solution, aspirate within 20 seconds
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Incubate 1–2 min at RT or 37°C; tap gently to dislodge
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Add 5 mL Neutralization Solution, centrifuge at 800g for 5 minutes
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Resuspend pellet and seed into 2–3 flasks (1:2 or 1:3 ratio)
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Maintenance
Change medium every 2–3 days. Cells typically reach confluency in ~7 days.
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Preparing Quiescent Cells
Once nearly confluent, switch to Endothelial Basal Medium for 12 hours before use in experiments.
Note: Use of non-Nuvyra Labs media or reagents may void performance guarantees.
Cells are offered for Research Use Only. Not for Clinical Use.
