Name of Products
Human Ovarian Microvascular Endothelial Cells
Catalog Number
HOM1
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
Human Ovarian Microvascular Endothelial Cells were isolated from normal human ovarian tissue and are supplied at passage 1. These cells are ideal for studying ovarian vasculature, angiogenesis, hormone-driven endothelial responses, and tissue-specific microvascular behavior. For best performance, we recommend culturing with Nuvyra Labs Endothelial Growth Medium.
Storage Conditions
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Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)
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Shipping Temperature: −80°C
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Stability: Long-term stability in cryogenic storage
Cell Characterization / Specification
Quality Control Results
| Parameter |
Method |
Specification |
Result |
| Sterility (Bacteria/Fungi) |
Growth Plate |
Negative |
Negative |
| Endotoxin Level |
Kinetic LAL |
≤1.0 EU/μg |
<0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / HBV (PCR) |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
>5 × 10⁵ |
5.0 × 10⁵ |
| Viability |
Trypan Blue |
>95% viable |
97% |
Handling Upon Arrival
Transfer vials to a −80°C freezer for short-term storage or a liquid nitrogen tank for long-term preservation.
Thawing & Subculturing Instructions
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Pre-coat flask: Add 2 mL Nuvyra Labs Coating Solution to a T25 flask, incubate 5 min, aspirate, and rinse with cell rinse solution.
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Thawing: Warm the vial in a 37°C water bath. Wipe with 70% ethanol and transfer cells to a biosafety cabinet.
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Seeding: Add 10 mL Nuvyra Labs Endothelial Growth Medium to pre-coated flask and transfer cells.
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Passaging:
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Rinse with rinse solution twice
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Add 2 mL Detachment Solution, aspirate after 20 sec
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Incubate 1–2 min until cells round up and detach
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Neutralize with 5 mL Neutralization Solution, centrifuge at 800g for 5 min
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Resuspend in fresh medium and split 1:2 or 1:3
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Feeding: Replace medium every 2–3 days
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Quiescence: For quiescent cells, switch to Endothelial Basal Medium for 12 hours before experiments
Note: Performance is only guaranteed with Nuvyra Labs reagents.
Cells are offered for Research Use Only. Not for Clinical Use.
