Name of Products
Human Liver Sinusoidal Microvascular Endothelial Cells
Catalog Number
HLS1
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
These endothelial cells were isolated from normal human liver tissue, specifically from the liver sinusoidal microvasculature. They provide an ideal model for studying hepatic endothelial biology, drug metabolism, liver-specific inflammation, and immune cell interactions. Supplied at passage 1, these cells are shipped frozen and should be handled with proper cryogenic precautions. For optimal results, we recommend culturing with Nuvyra Labs Endothelial Growth Medium.
Storage Conditions
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Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)
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Shipping Temperature: −80°C
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Stability: Long-term stable under cryogenic storage
Cell Characterization / Specification
Quality Control Data
| Parameter |
Method |
Acceptance Criteria |
Result |
| Sterility – Bacteria |
Growth Plate |
Negative |
Negative |
| Sterility – Fungi |
Growth Plate |
Negative |
Negative |
| Endotoxin Level |
Kinetic LAL |
≤1.0 EU/μg |
<0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / HBV (PCR) |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
>5 × 10⁵ |
5.0 × 10⁵ |
| Viability |
Trypan Blue |
>95% viable |
97% |
Handling Upon Arrival
Immediately store vials in a −80°C freezer for short-term use or liquid nitrogen for long-term storage.
Thawing & Subculturing Instructions
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Pre-coat T25 flask
Add 2 mL of Nuvyra Labs Coating Solution, incubate 5 min, aspirate, and rinse with 4 mL cell rinse solution.
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Thawing
Thaw frozen vial in a 37°C water bath, wipe with 70% ethanol, and transfer cells into a biosafety cabinet.
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Seeding
Add 10 mL of Nuvyra Labs Endothelial Growth Medium to the flask and transfer the cells.
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Passaging Protocol
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Rinse cells twice with 5 mL rinse solution
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Add 2 mL Detachment Solution, aspirate after 20 sec
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Incubate for 1–2 min until cells round up, tap to detach
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Add 5 mL Neutralization Solution, centrifuge at 800g for 5 min
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Resuspend pellet in medium and seed into 2–3 flasks at 1:2 or 1:3 ratio
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Medium Change
Replace medium every 2–3 days.
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Quiescent State Prep
When confluent, switch to Endothelial Basal Medium for 12 hours before experimentation.
Note: Product performance is only validated using Nuvyra Labs media and reagents.
Cells are offered for Research Use Only. Not for Clinical Use.
