Human Large Intestine Microvascular Endothelial Cells

Name of Products

Human Large Intestine Microvascular Endothelial Cells

Catalog Number

HL03

Product Format

Frozen Vial

Cell Number

5 × 10⁵ cells/vial

Product Description

Human Large Intestine Microvascular Endothelial Cells were isolated from healthy human colon tissue. The cells are provided at passage 1 and are ideal for intestinal vascular modeling, barrier function studies, and gut-liver axis research. For optimal performance, use with Nuvyra Labs Endothelial Growth Medium.

Storage

• Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)

• Shipping Temperature: −80°C

• Stability: Long-term stability under cryogenic storage

Cell Characterization / Specification

• VWF/Factor VIII Expression: >95% cytoplasmic positive (immunofluorescence)

• Di-I-Ac-LDL Uptake: >95% positive

• PECAM1 Expression: >95% positive (immunofluorescence)

Quality Control Results

Handling Upon Arrival

Immediately transfer frozen vials to −80°C for short-term storage or liquid nitrogen for long-term storage.

Thawing & Subculturing Instructions

1. Pre-coat flask: Add 2 mL Nuvyra Labs Coating Solution to a T25 flask. Incubate 5 min. Aspirate and rinse with cell rinse solution.

2. Thawing: Warm vial in 37°C water bath. Disinfect with 70% ethanol. Transfer cells into biosafety cabinet.

3. Seeding: Add 10 mL Nuvyra Labs Endothelial Growth Medium to pre-coated flask and seed cells.

4. Passaging:

   • Rinse flask twice with rinse solution

   • Add 2 mL Detachment Solution, aspirate after 20 sec

   • Wait 1–2 min, gently tap to dislodge cells

   • Add 5 mL Neutralization Solution, centrifuge 800g, 5 min

   • Resuspend and seed into 2–3 pre-coated flasks (1/2 to 1/3 ratio)

5. Feeding: Change medium every 2–3 days

6. Quiescence: When confluent, switch to Endothelial Basal Medium 12 hours before experiments

Note: Performance is only guaranteed with Nuvyra Labs reagents.

Cells are offered for Research Use Only. Not for Clinical Use.