Name of Products
Human Iliac Artery Endothelial Cells
Catalog Number
HLA1
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
Cells were isolated from normal human iliac artery tissue. They are shipped in frozen vials at passage 1. For optimal performance, we recommend culturing with Nuvyra Labs Endothelial Growth Medium.
Storage
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Storage Temperature: −196°C
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Shipping Temperature: −80°C
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Stability: Very stable in the Liquid Nitrogen Vapor Phase
Cell Characterization / Specification
Quality Control
| Parameter |
Method |
Acceptance Criteria |
Result |
| Bacterial Sterility |
Growth Plate |
Negative |
Negative |
| Fungal Sterility |
Growth Plate |
Negative |
Negative |
| Endotoxin Level (EU/mL) |
Kinetic LAL |
≤ 1.0 EU/μg |
< 0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / Hepatitis B (PCR) |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
> 5 × 10⁵ |
5.0 × 10⁵ |
| Viability (Trypan Blue Exclusion) |
Microscopy |
> 95% |
97% |
Handling Instructions Upon Arrival
Transfer the frozen vial into a −80°C freezer for short-term storage or a liquid nitrogen tank for long-term preservation.
Thawing and Subculturing Procedure
A) Pre-coat T25 flasks with 2 mL Nuvyra Labs Coating Solution, incubate 5 minutes. Aspirate, rinse with 4 mL of rinse solution, discard, and the flask is ready.
B) Thaw vial in a 37°C water bath. Sanitize the vial, then transfer cells to a biosafety cabinet. Add 10 mL of Nuvyra Labs Endothelial Growth Medium to the pre-coated flask.
C) Once confluent, rinse cells with 5 mL rinse solution (twice), then add 2 mL Nuvyra Labs Detachment Solution and aspirate after 20 seconds.
D) Incubate 1–2 minutes at RT or 37°C until cells round up. Tap flask to release cells.
E) Add 5 mL Nuvyra Labs Neutralization Solution, centrifuge at 800g for 5 minutes.
F) Resuspend in 10–15 mL medium and divide 5 mL each into 2–3 pre-coated flasks (1/2–1/3 ratio).
G) Change medium every 2–3 days; cells reach confluence in ~7 days.
H) For quiescent state preparation, switch to Nuvyra Labs Endothelial Basal Medium for ~12 hours.
Important Notes
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All human-derived products must be handled as potential biohazards.
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Use Nuvyra Labs reagents for validated growth and marker expression.
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Report quality concerns within 30 days of receipt for support.
Product Usage
Cells are offered for Research Use Only. Not for Clinical Use.
