Human Coronary Artery Endothelial Cells

Name of Product
Human Coronary Artery Endothelial Cells

Catalog Number 
HCA1

Product Format
Frozen Vial

Cell Number

5 × 10⁵ cells/vial

Product Description
Primary endothelial cells isolated from healthy human coronary artery tissue. These cells are shipped cryopreserved at passage 1 and exhibit strong endothelial phenotypes, including VWF, PECAM1, and DiI-Ac-LDL uptake. Recommended for vascular biology, drug screening, and cardiovascular research. Use with Nuvyra Labs Endothelial Growth Medium for optimal results.

Storage & Stability

• Storage Temperature: –196°C (Liquid Nitrogen Vapor Phase)

• Shipping Temperature: –80°C

• Stability: Stable long-term in LN₂ vapor phase when handled properly

Cell Characterization / Specification

• VWF/Factor VIII (Immunofluorescence): >95% cytoplasmic positive

• Di-I-Ac-LDL Uptake: >95% positive

• PECAM1 (CD31): >95% cytoplasmic positive

Quality Control

Handling Upon Arrival
Store in a –80°C freezer (short-term) or transfer immediately to liquid nitrogen for long-term storage.

Thawing & Subculturing Protocol

1. Pre-Coating Flask

   • Use 2 mL of Nuvyra Labs Coating Solution per T25 flask

   • Incubate 5 min at RT, rinse with 4 mL Cell Rinse Solution

2. Thawing

   • Thaw vial in 37°C water bath

   • Disinfect with 70% ethanol and proceed under sterile hood

   • Add contents to a pre-coated flask with 10 mL of Nuvyra Labs Endothelial Growth Medium

3. Passaging

   • Rinse cells with Cell Rinse Solution (×2)

   • Add 2 mL Nuvyra Labs Detachment Solution

   • Aspirate after 20 sec and wait 1–2 min until cells round up

   • Tap flask to dislodge cells

   • Neutralize with 5 mL Nuvyra Labs Neutralization Solution

   • Centrifuge at 800g for 5 min

4. Re-Seeding

   • Resuspend pellet in 10–15 mL growth medium

   • Seed 5 mL per flask (1:2 or 1:3 ratio)

   • Change medium every 2–3 days

5. To Prepare Quiescent Cells

   • Once confluent, switch to Nuvyra Labs Endothelial Basal Medium 12 hrs before experiment

Product Usage
Cells are offered for Research Use Only. Not for Clinical Use.