Human Cord Blood CD34⁺ Cells Derived Endothelial Cells

Name of Product
Human Cord Blood CD34⁺ Cells Derived Endothelial Cells

Catalog Number 
HC01

Product Format
Frozen Vial

Cell Number

5 × 10⁵ cells/vial

Product Description
These endothelial cells were derived from CD34-positive human cord blood cells cultured under endothelial-inducing conditions. The cells are supplied at passage 1 and cryopreserved in vials. For best performance, Nuvyra Labs Endothelial Growth Medium is recommended.

Storage & Stability

• Storage Temperature: –196°C (Liquid Nitrogen Vapor Phase)

• Shipping Temperature: –80°C

• Stability: Very stable in vapor phase; store long-term in LN₂

Cell Characterization / Specification

• VWF/Factor VIII (Cytoplasmic): >95% positive (Immunofluorescence)

• Di-I-Ac-LDL Uptake: >95% positive (Immunofluorescence)

• PECAM1 (CD31): >95% positive (Immunofluorescence)

Quality Control

Handling Upon Arrival
Transfer to –80°C for short-term or LN₂ tank for long-term storage.

Thawing & Subculturing Protocol

1. Pre-Coat Flask:
   Add 2 mL of Nuvyra Labs Coating Solution to a T25 flask. Incubate for 5 min. Aspirate and rinse with 4 mL Cell Rinse Solution. Flask is now ready.

2. Thawing:
   Thaw vial in a 37°C water bath. Wipe with 70% ethanol and transfer to biosafety cabinet. Add 10 mL of Endothelial Growth Medium to pre-coated flask and seed cells.

3. Passaging:

   • Rinse flask twice with 5 mL Cell Rinse Solution.

   • Add 2 mL Detachment Solution. Aspirate after 20 seconds.

   • Incubate 1–2 min at RT or 37°C. Tap flask if needed for detachment.

   • Add 5 mL Neutralization Solution, centrifuge at 800g for 5 min.

4. Seeding:
   Resuspend in 10–15 mL medium. Plate 5 mL per T25 flask (1:2 or 1:3 ratio).
   Change medium every 2–3 days. Confluence expected in ~7 days.

5. For Quiescent Cells:
   When nearly confluent, switch to Endothelial Basal Medium for 12 hours before experiments.

Product Usage
Cells are offered for Research Use Only. Not for Clinical Use.