Name of Product
Human Brain Microvascular Endothelial Cells Expressing VE-CAD-GFP
Catalog Number
HBM1
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
Human Brain Microvascular Endothelial Cells (HBMVECs) were isolated from normal human brain cortex tissue. Cells were genetically modified by lentiviral transduction to express VE-CAD-GFP and subsequently selected with puromycin. Cells are provided at passage 1 and shipped frozen. Nuvyra Labs Endothelial Growth Medium is strongly recommended for culturing these cells.
Storage
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Storage Temperature: –196°C (liquid nitrogen vapor phase)
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Shipping Temperature: –80°C (dry ice)
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Stability: Very stable in liquid nitrogen vapor phase
Cell Characterization / Specification
Quality Control & Safety Testing
| Parameter |
Method |
Criteria |
Result |
| Sterility (Bacteria/Fungi) |
Growth Plate |
Negative |
Negative |
| Endotoxin Level |
Kinetic LAL |
≤1.0 EU/μg |
<0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1, HIV-2, HBV |
PCR |
Negative |
Negative |
| Cell Count |
Manual |
>5 × 10⁵ |
5.0 × 10⁵ |
| Viability (Trypan Blue) |
Trypan Blue Excl. |
>95% |
97% |
Handling Upon Arrival
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Store in liquid nitrogen vapor phase for long-term use.
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For short-term storage, vials can be kept at –80°C.
Thawing and Subculturing Instructions
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Pre-Coating: Add 2 mL of Nuvyra Labs Coating Solution to a T25 flask, incubate 5 min at RT. Rinse with 4 mL cell rinse solution.
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Thawing: Thaw vial in a 37°C water bath. Wipe with 70% ethanol. Transfer to biosafety cabinet.
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Seeding: Add 10 mL Nuvyra Labs Endothelial Growth Medium to flask and add thawed cells.
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Passaging: Rinse flask with 5 mL rinse solution twice. Add 2 mL detachment solution. After 1–2 minutes, tap flask gently to release cells.
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Neutralization: Add 5 mL Nuvyra Labs Neutralization Solution. Centrifuge at 800 × g for 5 minutes.
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Expansion: Resuspend in 10–15 mL medium. Seed 5 mL per pre-coated T25 flask (1:2–1:3 ratio).
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Medium Change: Every 2–3 days. Confluence typically reached in 7 days.
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Quiescence Preparation: Switch to Nuvyra Labs Endothelial Basal Medium for 12 hours prior to experiments when cells are nearly confluent.
Product Usage
Cells are offered for Research Use Only. Not for Clinical Use.
