Human Aortic Artery Endothelial Cells

Name of Product
Human Aortic Artery Endothelial Cells

Catalog Number 
HAE1

Product Format
Frozen Vial

Cell Number

5 × 10⁵ cells/vial

Product Description
Human Aortic Artery Endothelial Cells were isolated from normal human aortic tissue and are provided at passage 1 in cryopreserved vials. These cells are ideal for vascular biology studies, inflammation research, drug permeability assays, and more. Nuvyra Labs Endothelial Growth Medium is recommended for optimal culture performance.

Storage

• Storage Temperature: –196°C (liquid nitrogen vapor phase)

• Shipping Temperature: –80°C (dry ice)

• Stability: Very stable in liquid nitrogen vapor phase

Cell Characterization / Specification

• Cytoplasmic VWF / Factor VIII: >95% positive (Immunofluorescence)

• Cytoplasmic Di-I-Ac-LDL Uptake: >95% positive (Immunofluorescence)

• Cytoplasmic PECAM1 (CD31): >95% positive (Immunofluorescence)

Quality Control and Testing

Handling Upon Arrival
Store in a –80°C freezer for short-term storage or transfer to a liquid nitrogen tank for long-term preservation.

Thawing and Subculturing Protocol

1. Pre-Coating Flask
   Add 2 mL Universal Coating Solution to a T25 flask. Incubate at room temperature for 5 minutes. Discard excess solution and rinse with 4 mL of Cell Rinse Solution. Remove PBS and proceed to seeding.

2. Thawing Cells
   Thaw the frozen vial in a 37°C water bath. Wipe the vial with 70% ethanol before transferring to a biosafety cabinet. Seed cells into a pre-coated T25 flask containing 10 mL of Nuvyra Labs Endothelial Growth Medium.

3. Passaging

   • Rinse with 5 mL Cell Rinse Solution twice.

   • Add 2 mL Nuvyra Labs Detachment Solution and aspirate excess after 20 seconds.

   • Incubate at RT or 37°C for 1–2 minutes. Tap flask gently to dislodge cells.

   • Add 5 mL Nuvyra Labs Neutralization Solution. Centrifuge at 800g for 5 minutes.

4. Expansion

   • Resuspend pellet in 10–15 mL medium.

   • Transfer 5 mL into each of 2–3 pre-coated T25 flasks (1:2–1:3 ratio).

   • Change medium every 2–3 days. Cells typically reach confluency in 7 days.

5. Preparing Quiescent Cells
   When nearly confluent, switch to Nuvyra Labs Endothelial Basal Medium for 12 hours before experiments.

Product Usage
Cells are offered for Research Use Only. Not for Clinical Use.