Name of Product
DMEM High Glucose, With L-Glutamine, With Sodium Pyruvate, With Pyridoxine HCl
Catalog Number
DN96
Product Format
Liquid
Volume: 1x500 mL
Product Description
Dulbecco’s Modified Eagle Medium (DMEM) High Glucose is a nutrient-rich formulation containing L-glutamine, sodium pyruvate, and pyridoxine HCl. With a glucose concentration of approximately 4.5 g/L, it is designed to support the growth and maintenance of energy-demanding cells such as cancer cells, primary cells, and stem cells.
Applications
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Cell Culture: Supports proliferation of fibroblasts, HeLa cells, and other primary and immortalized mammalian cells
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Research: Commonly used in studies involving cell proliferation, metabolism, differentiation, and response to environmental stimuli
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Stem Cell Culture: Ideal for culturing iPSCs and other stem cells that require high nutrient density
Supplementation Recommendations
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FBS: Typically 5–10% for essential nutrients and growth factors
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Optional Additions: Antibiotics, non-essential amino acids, or specific growth factors as needed for experimental conditions
Glutamine Stability Notice
L-glutamine can degrade over time, especially at 37°C. Regularly check the expiration date and supplement with fresh L-glutamine as required.
Storage and Handling
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Storage: 2–8°C in the dark
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Shelf Life: Use before expiration date
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Handling: Use sterile techniques to avoid contamination
Product Usage
Cells are offered for Research Use Only. Not for Clinical Use.
