DMEM 1X, High Glucose, With L-Glutamine, Without Sodium Pyruvate, Without HEPES (1x500ml bottles)

Name of Product
DMEM 1X, High Glucose, With L-Glutamine, Without Sodium Pyruvate, Without HEPES

Catalog Number
DM03

Product Format
Liquid
Volume: 500 mL

Product Description
This DMEM (Dulbecco’s Modified Eagle Medium) formulation is a widely used basal medium designed to support the growth of various adherent and suspension cell lines. It contains high glucose and L-glutamine, but excludes sodium pyruvate and HEPES, making it suitable for a 5–10% CO₂ incubator environment to maintain physiological pH (~7.4).

Applications
This formulation supports the proliferation and maintenance of:

• Fibroblasts

• Neurons

• Certain cancer cell lines

It is frequently used in:

• General cell culture

• Transfection studies

• Recombinant protein expression

Supplementation Recommendations
Prior to use, this medium is commonly supplemented with:

• Fetal Bovine Serum (FBS): Typically 5–10% for optimal cell growth

• Antibiotics: Such as penicillin-streptomycin to reduce contamination risk

CO₂ Requirement
Since this formulation does not contain HEPES buffer, it requires a controlled environment of 5–10% CO₂ to maintain pH balance.

L-Glutamine Consideration
L-glutamine is known to degrade in solution at 37°C. For long-term cultures, consider adding fresh L-glutamine at each passage or using a stabilized substitute such as GlutaMAX.

Storage and Handling

• Storage: 2–8°C in the dark

• Shelf Life: 6–12 months unopened

• Thawing: Thaw at 2–8°C or in a 37°C water bath

• Avoid: Repeated freeze-thaw cycles

Product Usage
Cells are offered for Research Use Only. Not for Clinical Use.