Immortalized Brain Vascular Fibroblast Growth Medium

Immortalized Brain Vascular Fibroblast Growth Medium

$650.00

Catalog Number: IB01
Product Format: Frozen or Liquid
Product Volume: 500 mL
Product Usage: Research Use Only. Not for Clinical Use.

Custom Formulations Available

  • Neurovascular niche medium (with astrocyte-conditioned factors)

  • Hypoxia-adapted (1% O₂ formulation)

Product Description

A specialized medium optimized for the culture and expansion of immortalized brain vascular fibroblasts (e.g., hBVFs, HBVP-immortalized lines). This formulation supports the unique metabolic and functional needs of cerebrovascular stromal cells, preserving blood-brain barrier (BBB) relevance and promoting angiogenic potential.

Physicochemical Properties

  • pH: 7.2–7.4

  • Osmolality: 310–330 mOsm/kg

  • Color: Red (contains phenol red indicator)

Key Features

✔ Validated for immortalized brain vascular fibroblasts
✔ Maintains BBB-relevant markers:

  • CD13⁺, PDGFR-β⁺, α-SMA⁺ (flow cytometry)

  • Collagen IV and laminin secretion (ICC/ELISA)
    ✔ Supports angiogenesis assays: tube formation, endothelial co-culture
    ✔ Pre-screened: mycoplasma, virus, sterility
    ✔ Available in serum-free or low-serum options

Storage & Handling

  • Liquid Storage: 2–8°C (up to 12 months unopened)

  • Frozen Storage: –20°C (for long-term storage)

  • After Opening: Use within 4 weeks if stored at 2–8°C

  • Avoid freeze-thaw cycles

Recommended Culture Protocol

Subculture Procedure

  1. Wash cells with PBS (Ca²⁺/Mg²⁺-free)

  2. Detach with cell detachment solution

  3. Neutralize with neutralization buffer

  4. Centrifuge at 200 × g for 5 minutes

  5. Resuspend in fresh IBVF medium and seed at 5,000–10,000 cells/cm² in T25 or T75 flasks

Feeding Schedule

  • Replace medium every 48–72 hours

  • Passage cells at 70–80% confluence (avoid overgrowth)

Quality Control – Performance Specifications

Parameter Specification
Viability (Trypan Blue) ≥ 95%
Doubling Time 30–36 hours
PDGFR-β Expression (FC) ≥ 90% positive
VEGF Secretion (ELISA) 200–500 pg/mL/24h

Safety Testing:

  • Sterility (14-day incubation): No microbial growth

  • Mycoplasma (PCR): Negative

Troubleshooting Guide

Issue Potential Cause Solution
Poor cell attachment Insufficient matrix coating Use collagen IV or fibronectin
Reduced growth rate Growth factor degradation Use fresh bFGF aliquots
Morphological changes High passage number Use cells between P5–P20

Ordering Information

Size Catalog No.
500 mL IBVF-GM500
1 L IBVF-GM1000

References

  • Nakagawa et al., J Cereb Blood Flow Metab (2009) – hBVF isolation

  • Sweeney et al., Nature Neuroscience (2019) – fibroblast role in BBB integrity

Related Products

  • Brain Vascular Fibroblast Cryopreservation Medium

  • Fibroblast Activation Marker Antibodies

Product Usage

Cells are offered for Research Use Only. Not for Clinical Use.

Benefits

Maximizes Cell Viability & Recovery

Preserves Phenotype & Biomarker Expression

Immortalized Brain Vascular Fibroblast Growth Medium
  • Immortalized Brain Vascular Fibroblast Growth Medium
  • Immortalized Brain Vascular Fibroblast Growth Medium
  • Immortalized Brain Vascular Fibroblast Growth Medium
  • Immortalized Brain Vascular Fibroblast Growth Medium

Scientifically engineered formulations, optimized for maximum cellular performance and validated through rigorous quality control.

Optimized for cellular viability and function

Engineered to support robust cell health, proliferation, and performance across passages.
Formulated for consistency and reproducibility

Minimizes variability to ensure reliable outcomes in every experiment.
Validated for purity, sterility, and performance

Extensively tested to meet rigorous quality, safety, and regulatory standards.

Stabilized

Broad cell line compatibility

Scientifically validated

Nutrient-optimized

Consistency you can trust. Each formulation is optimized to deliver reliable outcomes across experiments and timepoints.

Pairs well with