Human Saphenous Vein Endothelial Cells

Name of Products

Human Saphenous Vein Endothelial Cells

Catalog Number

HSV1

Product Format

Frozen Vial

Cell Number

5 × 10⁵ cells/vial

Product Description

Cells were isolated from normal human saphenous vein tissue, providing a relevant model for vascular biology, thrombosis, and endothelial barrier studies. Cells are shipped frozen at passage 1. Nuvyra Labs Endothelial Growth Medium is strongly recommended to maintain optimal morphology and proliferation.

Storage Conditions

• Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)

• Shipping Temperature: −80°C

• Stability: Very stable when stored properly

Cell Characterization / Specification

• VWF/Factor VIII: >95% positive (cytoplasmic, immunofluorescence)

• Di-I-Ac-LDL Uptake: >95% positive (cytoplasmic)

• PECAM1: >95% positive (cytoplasmic)

Quality Control Summary

Handling Upon Arrival

• Transfer immediately into −80°C freezer (short term) or liquid nitrogen (long-term).

• Do not thaw unless ready for use.

Thawing & Subculturing Instructions

A) Pre-coat T25 flask with 2 mL Nuvyra Labs Coating Solution, incubate 5 min at room temp, rinse with 4 mL cell rinse solution.
B) Thaw cell vial in 37°C water bath, disinfect exterior with 70% ethanol, and transfer into biosafety cabinet.
C) Add 10 mL Endothelial Growth Medium into flask and transfer cells.
D) When confluent, passage as follows:

• Rinse with 5 mL rinse solution (×2)

• Add 2 mL Detachment Solution, aspirate excess in 20 seconds

• Wait 1–2 min at RT or 37°C, tap to release cells

• Add 5 mL Neutralization Solution and centrifuge at 800g × 5 min

E) Resuspend in 10–15 mL medium, seed 5 mL/flask into 2–3 pre-coated flasks (1:2 or 1:3 split)
F) Change medium every 2–3 days; cells become confluent within 7 days
G) For quiescent cells: switch to Endothelial Basal Medium for 12 hrs pre-experiment

Cells are offered for Research Use Only. Not for Clinical Use.