Name of Products
Human Internal Mammary Artery Endothelial Cells
Catalog Number
HIM2
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
Cells were isolated from normal human internal mammary artery tissue and are provided at passage 1 in frozen vials. Recommended culture conditions include Nuvyra Labs Endothelial Growth Medium to ensure optimal viability and marker fidelity.
Storage
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Storage Temperature: −196°C (Liquid Nitrogen Vapor Phase)
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Shipping Temperature: −80°C
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Stability: Very stable during long-term cryogenic storage
Cell Characterization / Specification
Quality Control
| Parameter |
Method |
Acceptance Criteria |
Result |
| Bacterial Sterility |
Growth Plate |
Negative |
Negative |
| Fungal Sterility |
Growth Plate |
Negative |
Negative |
| Endotoxin Level (EU/mL) |
Kinetic LAL |
≤ 1.0 EU/μg |
< 0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / Hepatitis B (PCR) |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
> 5 × 10⁵ |
5.0 × 10⁵ |
| Viability (Trypan Blue Exclusion) |
Microscopy |
> 95% |
97% |
Handling Instructions Upon Arrival
Transfer frozen vial into −80°C freezer for short-term or liquid nitrogen tank for long-term storage.
Thawing & Subculturing Protocol
A) Pre-coat T25 flasks using 2 mL Nuvyra Labs Coating Solution, incubate for 5 minutes, rinse with 4 mL rinse solution.
B) Thaw vial at 37°C, sanitize, and transfer to biosafety cabinet. Add 10 mL Nuvyra Labs Endothelial Growth Medium to pre-coated flask and plate cells.
C) When confluent, rinse cells twice with rinse solution, then apply 2 mL Nuvyra Labs Detachment Solution and aspirate after 20 seconds.
D) Wait 1–2 minutes or until cells round up, then tap flask to detach.
E) Add 5 mL Nuvyra Labs Neutralization Solution, centrifuge at 800g for 5 min.
F) Resuspend in 10–15 mL fresh medium, seed into 2–3 T25 flasks (1/2 or 1/3 ratio).
G) Feed every 2–3 days. Confluency usually reached in 7 days.
H) For quiescence, switch to Endothelial Basal Medium ~12 hours before experiments.
Important Notes
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Use only Nuvyra Labs reagents to ensure consistent cell phenotype and performance.
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Report any quality-related concerns within 30 days of receipt.
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Always treat human-derived materials as potential biohazards.
Intended Use
For Research Use Only. Not for Clinical Use.
