Name of Products
Human Glomerular Microvascular Endothelial Cells
Catalog Number
HG01
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
Cells were isolated from normal human kidney glomeruli. Cells are shipped in frozen vials (provided at passage 1). Nuvyra Labs Endothelial Growth Medium is recommended for culturing these cells.
Storage
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Storage Temperature: −196°C
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Shipping Temperature: −80°C
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Stability: Very stable in the liquid nitrogen vapor phase
Cell Characterization / Specification
Quality Control
| Parameter |
Method of Determination |
Acceptance Criteria |
Result |
| Sterility Test |
Growth Plate |
Negative |
Negative |
| Fungi |
Growth Plate |
Negative |
Negative |
| Endotoxin Level (EU/mL) |
Kinetic LAL |
≤ 1.0 EU/μg |
< 0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / HBV (PCR) |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
> 5 × 10⁵ |
5.0 × 10⁵ |
| Viability (Trypan Blue Exclusion) |
Microscopy |
> 95% |
97% |
Handling Cells Upon Arrival
Transfer frozen vials to a −80°C freezer (short-term) or liquid nitrogen tank (long-term).
Thawing and Subculturing Protocol
A) Pre-coat T25 flasks with 2 mL of Nuvyra Labs Coating Solution, incubate 5 min at room temperature. Aspirate and rinse with 4 mL cell rinse solution. Discard.
B) Thaw vial in 37°C water bath, wipe with 70% ethanol, and transfer to biosafety cabinet. Add to flask with 10 mL Nuvyra Labs Endothelial Growth Medium.
C) For passaging:
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Rinse with 5 mL cell rinse solution twice.
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Add 2 mL Nuvyra Labs Detachment Solution, aspirate after 20 sec.
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Leave flask at RT or 37°C for 1–2 min. Gently tap to detach cells.
D) Add 5 mL Nuvyra Labs Neutralization Solution, centrifuge at 800 × g for 5 min.
E) Resuspend pellet in 10–15 mL medium and seed 5 mL into 2–3 coated flasks (1/2 to 1/3 ratio).
F) Change medium every 2–3 days. Cells typically reach confluence within 7 days.
G) To prepare quiescent cells, replace growth medium with Nuvyra Labs Endothelial Basal Medium for 12 hours prior to experiments.
Important Notes
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Human tissue-derived products are potentially biohazardous. Use proper PPE.
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Use only Nuvyra Labs reagents to ensure product specifications are met.
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Quality-related claims must be submitted within one month of receipt.
Product Usage
Cells are offered for Research Use Only. Not for Clinical Use.
