Name of Products
Human Dermal Lymphatic Microvascular Endothelial Cells
Catalog Number
HDL1
Product Format
Frozen Vial
Cell Number
5 × 10⁵ cells/vial
Product Description
Human Dermal Lymphatic Microvascular Endothelial Cells were isolated from normal human skin. Cells are shipped in frozen vials (provided at passage 1). Nuvyra Labs Endothelial Growth Medium is recommended for culturing these cells.
Storage
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Storage Temperature: −196°C
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Shipping Temperature: −80°C
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Stability: Very stable in the Liquid Nitrogen Vapor Phase
Cell Characterization / Specification
Quality Control
| Parameter |
Method of Determination |
Acceptance Criteria |
Result |
| Sterility Test |
Growth Plate |
Negative |
Negative |
| Fungi |
Growth Plate |
Negative |
Negative |
| Endotoxin Level (EU/mL) |
Kinetic LAL |
≤ 1.0 EU/μg |
< 0.05 EU/μg |
| Mycoplasma (PCR) |
PCR |
Negative |
Negative |
| HIV-1 / HIV-2 / HBV (PCR) |
PCR |
Negative |
Negative |
| Cell Count |
Manual Count |
> 5 × 10⁵ |
5.0 × 10⁵ |
| Viability (Trypan Blue Exclusion) |
Microscopy |
> 95% |
97% |
Handling Cells Upon Arrival
Transfer frozen vials to a −80°C freezer (short-term) or liquid nitrogen tank (long-term).
Thawing and Subculturing Protocol
A) Pre-coat T25 flasks with 2 mL of Nuvyra Labs Coating Solution, incubate 5 minutes at room temperature. Aspirate and rinse with 4 mL of cell rinse solution. Discard PBS; flask is ready.
B) Thaw the vial in a 37°C water bath. Spray with 70% ethanol and wipe before opening in a biosafety cabinet. Add 10 mL of Nuvyra Labs Endothelial Growth Medium to a pre-coated flask and transfer the cell suspension.
C) To passage cells:
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Rinse with 5 mL of cell rinse solution twice.
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Add 2 mL of Nuvyra Labs Detachment Solution and aspirate within 20 seconds.
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Leave at RT or 37°C for 1–2 minutes. Tap flask to release cells.
D) Add 5 mL of Nuvyra Labs Neutralization Solution, centrifuge at 800 × g for 5 minutes.
E) Resuspend the pellet in 10–15 mL medium and seed 5 mL into 2–3 pre-coated T25 flasks (1/2 to 1/3 subculture ratio).
F) Change medium every 2–3 days. Cells typically reach confluence within 7 days.
G) For quiescent cells, replace growth medium with Nuvyra Labs Endothelial Basal Medium for ~12 hours before experiments.
Important Notes
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Human tissue-derived products are potentially biohazardous. Follow standard biosafety precautions.
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Use only Nuvyra Labs reagents to ensure growth and marker fidelity.
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Quality concerns must be reported within one month of delivery. Replacements are not guaranteed beyond that period.
Product Usage
Cells are offered for Research Use Only. Not for Clinical Use.
