Human Cardiac Microvascular Endothelial Cells

Name of Product
Human Cardiac Microvascular Endothelial Cells

Catalog Number 
HCM1

Product Format
Frozen Vial

Cell Number

5 × 10⁵ cells/vial

Product Description
Human Cardiac Microvascular Endothelial Cells were isolated from normal human cardiac tissue and are provided at passage 1 in cryopreserved vials. These cells are suitable for cardiovascular research, drug screening, and endothelial function studies. Nuvyra Labs Endothelial Growth Medium is recommended for optimal growth and viability.

Storage

• Storage Temperature: –196°C (liquid nitrogen vapor phase)

• Shipping Temperature: –80°C (dry ice)

• Stability: Very stable in liquid nitrogen vapor phase

Cell Characterization / Specification

• Cytoplasmic VWF / Factor VIII Expression: >95% positive (Immunofluorescence)

• Cytoplasmic Di-I-Ac-LDL Uptake: >95% positive (Immunofluorescence)

• Cytoplasmic PECAM1 (CD31) Expression: >95% positive (Immunofluorescence)

Quality Control and Testing

Handling Upon Arrival
Transfer vials into a –80°C freezer for short-term storage or liquid nitrogen tank for long-term storage.

Thawing and Subculturing Protocol

1. Pre-Coating Flask
   Add 2 mL Universal Coating Solution to a T25 flask, incubate for 5 min, discard excess, rinse with 4 mL Cell Rinse Solution, and remove PBS.

2. Thawing Cells
   Thaw the vial in a 37°C water bath. Wipe with 70% ethanol, transfer to a biosafety cabinet, and add 10 mL Nuvyra Labs Endothelial Growth Medium to a pre-coated T25 flask.

3. Passaging Cells

   • Rinse flask with 5 mL Cell Rinse Solution twice.

   • Add 2 mL Nuvyra Labs Detachment Solution and aspirate excess after 20 seconds.

   • Incubate at RT or 37°C for 1–2 minutes. Tap to detach cells.

   • Add 5 mL Nuvyra Labs Neutralization Solution and centrifuge at 800g for 5 minutes.

4. Expansion
   Resuspend in 10–15 mL medium. Plate 5 mL each into 2–3 flasks (1:2–1:3 ratio). Change medium every 2–3 days. Cells typically reach confluency in 7 days.

5. Quiescent Cell Prep
   Replace growth medium with Nuvyra Labs Endothelial Basal Medium for 12 hours prior to experiments.

Product Usage
Cells are offered for Research Use Only. Not for Clinical Use.