Name of Product
DMEM/F-12 Ham 1:1 Mixture, 1X, With L-Glutamine, With HEPES, Without Sodium Bicarbonate
Catalog Number
DN22
Product Format
Liquid
Volume: 500 mL
Product Description
DMEM/F-12 Ham 1:1 Mixture is a versatile and well-established cell culture medium composed of equal parts Dulbecco's Modified Eagle Medium (DMEM) and Ham’s F-12 Nutrient Mixture. This formulation is optimized to support a broad range of mammalian cell types, including primary cells and established cell lines, in both adherent and suspension cultures.
Applications
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Suitable for culturing primary cells, stem cells, and hybridomas
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Effective for cell proliferation assays, differentiation studies, and cytotoxicity testing
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Widely used in drug development and cancer research models
Supplementation Guidelines
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Serum: Supplement with Fetal Bovine Serum (FBS) or equivalent to support cell viability and growth
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Antibiotics: Addition of penicillin-streptomycin helps prevent microbial contamination
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Growth Factors: Specialized additives may be required for cell-specific growth or differentiation protocols
Storage Instructions
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Temperature: Store at 2–8°C
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Protection: Shield from light to preserve light-sensitive components
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Freezing: Avoid freezing to prevent precipitation of components and loss of performance
Handling Note
Aseptic technique is critical to avoid contamination and preserve the integrity of your cell culture work.
Product Usage
Cells are offered for Research Use Only. Not for Clinical Use.
